RT-PCR + Conventional PCR
Course Description
🧬 RT-PCR & Conventional PCR with Gel Electrophoresis – Comprehensive Hands-On Training
🔍 Course Overview
This intensive course offers a deep dive into the foundational and advanced techniques of molecular biology, focusing on both Conventional PCR and Real-Time PCR (RT-PCR) methodologies. Participants will gain practical experience in DNA amplification, RNA analysis, and the visualization of nucleic acids using gel electrophoresis.
🎯 Learning Objectives
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Understand Fundamental Principles: Grasp the core concepts of Conventional PCR and RT-PCR, including their mechanisms and applications in molecular diagnostics.
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Hands-On Laboratory Skills: Perform DNA and RNA extraction, set up PCR reactions, and analyze results through agarose gel electrophoresis.
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Data Interpretation: Learn to interpret amplification curves, understand Ct values in RT-PCR, and troubleshoot common issues.
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Application in Research and Diagnostics: Apply techniques in real-world scenarios such as gene expression studies, pathogen detection, and genetic variation analysis.Manipal
🧪 Course Modules
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Introduction to Molecular Techniques
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Basics of nucleic acid structure and function
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Overview of PCR technologiesGenetech+1Wikipedia+1
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Conventional PCR Techniques
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Primer design and reaction setup
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Thermal cycling parameters
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Agarose gel preparation and electrophoresis
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Visualization and analysis of DNA bandsRoyal Society of ChemistryWikipedia+2Advance Biotechnology Centre+2base-asia.com+2
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Real-Time PCR (RT-PCR)
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Reverse transcription of RNA to cDNA
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Quantitative PCR setup and execution
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Fluorescent dye usage and data acquisition
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Interpretation of real-time amplification dataWikipediaRoyal Society of Chemistry
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Troubleshooting and Optimization
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Common pitfalls in PCR and RT-PCR
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Strategies for improving reaction efficiency
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Quality control measuresbase-asia.com+6IML Research+6Royal Society of Chemistry+6
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📅 Course Details
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Duration: 1 Week (3 Days Online Theory, 2 Days Laboratory Practicals)
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Mode: Hybrid (Online and In-Person)
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Location: Skillcare.xyz Training Facility
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Fee: ৳6,000Royal Society of Chemistry+4bioiconacademy.org+4en.hcxfmusp.org.br+4en.hcxfmusp.org.br+1invitro-experts.com+1
👥 Who Should Enroll?
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Undergraduate and postgraduate students in biotechnology, microbiology, or related fields
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Laboratory technicians and research assistants
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Healthcare professionals interested in molecular diagnostics
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Anyone seeking practical skills in PCR and RT-PCR techniquesManipalbioiconacademy.org
📝 Certification
Upon successful completion, participants will receive a certificate accredited by Skillcare.xyz, recognizing their proficiency in PCR and RT-PCR methodologies.
Course Outcomes
Upon successful completion of this comprehensive training, participants will be able to:
🧬 Theoretical Knowledge
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Understand Fundamental Principles: Articulate the core concepts of Conventional PCR and RT-PCR, including their mechanisms and applications in molecular diagnostics.
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Differentiate PCR Techniques: Distinguish between Conventional PCR and Real-Time PCR methodologies, understanding their respective advantages, limitations, and applications. Comprehend Gel Electrophoresis: Explain the principles of agarose gel electrophoresis and its role in analyzing PCR products.
🧪 Practical Skills
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Execute PCR Protocols: Perform DNA and RNA extraction, set up PCR reactions, and run thermal cycler programs for both Conventional PCR and RT-PCR.
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Conduct Gel Electrophoresis: Prepare and run agarose gels to separate and visualize DNA fragments, interpreting band patterns to assess PCR results. Design Primers: Design specific primers for target genes, optimizing annealing temperatures and reaction conditions for efficient amplification.
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Interpret Amplification Data: Analyze amplification curves and Ct values in RT-PCR to quantify gene expression levels.
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Troubleshoot Experiments: Identify and resolve common issues in PCR experiments, such as non-specific amplification, primer-dimer formation, and weak signals.
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Ensure Quality Control: Implement quality assurance measures and standard operating procedures to maintain the integrity and reliability of molecular diagnostic tests.
💼 Professional Competence
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Apply Techniques in Real-World Scenarios: Utilize acquired skills in research, clinical diagnostics, and biotechnology industries, contributing effectively to laboratory operations.
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Adhere to Ethical Standards: Demonstrate professional conduct and ethical considerations in molecular biology practices, including data handling and reporting.
Course Faq
1. What is the structure of the course?
The course is a hybrid program combining both theoretical and practical components. It includes: Online Theory Sessions: Three days of interactive online lectures covering the fundamentals of PCR techniques. Hands-On Laboratory Practicals: Two days of in-person laboratory sessions focusing on DNA/RNA extraction, PCR setup, and gel electrophoresis procedures.
2. Who is the target audience for this course?
This course is designed for: Undergraduate and postgraduate students in biotechnology, microbiology, or related fields. Laboratory technicians and research assistants seeking to enhance their molecular biology skills. Healthcare professionals interested in molecular diagnostics. Individuals aiming to acquire practical experience in PCR and gel electrophoresis techniques.
Hardson Smith
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