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Hands-on Training in RT-PCR: Techniques and Applications in Molecular Diagnostics and others

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6 Lessons
Duration : 07:21:12
8000.00 ৳

Hands-on Training in RT-PCR: Techniques and Applications in Molecular Diagnostics and others

Course Description

This practical training offers in-depth exposure to Real-Time PCR (RT-PCR) techniques used in molecular diagnostics, gene expression analysis, and disease detection. Participants will gain hands-on experience in RNA extraction, cDNA synthesis, and real-time amplification with data interpretation relevant to research, healthcare, and biotechnology.

Course Outcomes

  1. Understand the Principles of RT-PCR

    • Grasp the fundamentals of reverse transcription and real-time amplification processes.

  2. Master RNA Extraction & cDNA Synthesis

    • Perform RNA isolation from biological samples and convert RNA into complementary DNA (cDNA).

  3. Operate Real-Time PCR Instruments

    • Learn to set up and run RT-PCR experiments using real-time thermal cyclers and fluorescence detection.

  4. Analyze and Interpret Amplification Data

    • Gain skills in threshold cycle (Ct) analysis, melt curve evaluation, and gene expression quantification.

  5. Apply RT-PCR in Diagnostics & Research

    • Understand how RT-PCR is used in disease detection, pharmacogenomics, and molecular biology research.

  6. Ensure Quality and Accuracy

    • Learn about controls, standard curves, and data validation techniques for reliable results.

  7. Enhance Laboratory Reporting Skills

    • Improve documentation, result interpretation, and reporting aligned with diagnostic standards.

  8. Earn a Certificate of Completion

    • Receive a recognized certificate, validating your practical competency in RT-PCR techniques.

Course Faq

1. What is this training about?

This training focuses on teaching the fundamentals and lab techniques of Conventional PCR (Polymerase Chain Reaction) and Gel Electrophoresis for DNA analysis and visualization.

2. Who should attend this course?

Students, researchers, lab technologists, and anyone interested in molecular biology or genetic diagnostics can benefit from this course.

3. Do I need any prior lab experience?

No advanced experience is required. Basic understanding of biology or genetics is helpful but not mandatory. The course is beginner-friendly and guided.

4. What will I learn practically?

You will learn to: Prepare and handle PCR reagents Operate thermal cyclers Perform agarose gel preparation and loading Run and visualize DNA bands Troubleshoot errors and interpret results

5. What is the duration of the training?

The exact duration will be announced soon, but it typically spans 1–2 days of intensive hands-on lab sessions.

6. Will I receive a certificate?

Yes, all participants will receive a recognized certificate upon successful completion of the training.

7. Where will the training be held?

The training will be conducted at a fully equipped molecular biology lab in Dhaka, managed by BioIcon Academy (PBS Group).

8. How can I register or get more information?

Visit bioiconacademy.org or contact: 📞 +880 1896-061751 📩 Message us on Facebook or WhatsApp

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Hardson Smith

a week ago

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