Bundle Course (3 in 1) : RT-PCR: Techniques and Applications + Conventional PCR with Gel Electrophoresis Technique+ Pharmacogenomics Screening

Module-1 (BC-C02-M01): Overview and Application of Conventional PCR in Research & Diagnostics

Overview of molecular biology techniques, Status and prospect of molecular biology field in Bangladesh and career guideline, Application of Conventional PCR in Research & Diagnostics 0h 42m 09s
Module-2 (BC-C02-M02): Fundamental of PCR techniques and overview of PCR Lab

Fundamental of PCR techniques, Setting up a PCR lab: equipment, consumables and Layout 1h 42m 09s
Module-3 (BC-C02-M03): Biosafety and Quality Control for Molecular Diagnostics Lab

Biosafety Level & Biosecurity for Molecular Diagnostics Lab, Quality assurance & Trouble shooting of molecular diagnosis 0h 39m 12s
Module-4(BC-C02-M04): Course Final Assessment

Course Final Assessment 0h 10m 00s
Module-5(BC-C02-M05): Overview of molecular diagnosis in Bangladesh and Career guideline

Overview of molecular diagnosis, Status and prospect of molecular diagnosis field in Bangladesh and Career guideline 1h 22m 01s
Module-6(BC-C02-M06): Fundamental of conventional PCR & RT PCR and Lab Setting

Fundamental of conventional PCR & RT PCR, Setting up a RT-PCR lab: equipment, consumables and Layout 1h 34m 04s
Module-7(BC-C02-M07): Biosafety, sample processing, nucleic acid extraction and RT-PCR Run

Biosafety guideline, patient profile, sample collection, transport and preservation, Practical training on sample processing, nucleic acid extraction and master mix preparation, PCR profiling, result analysis and trouble shooting, COVID-19 RT-PCR detection 2h 21m 59s
Module-8(BC-C02-M08): RT-PCR based test parameters for molecular diagnosis

Introduction of others test parameter for molecular diagnosis, Conventional PCR use in Molecular Diagnosis (Pharmacogenomics & Tissue Typing) 1h 13m 56s
Module-9(BC-C02-M09): QC & QA of molecular diagnosis

Quality assurance & Trouble shooting of molecular diagnosis, Professional code of conduct & ethics for molecular diagnosis 0h 39m 12s
Module-10(BC-C02-M10): Course Final Assessment

Course Final Assessment 0h 10m 00s
Module-11(BC-C02-M11): Basic of Medical Molecular Genetics

Basic of Medical Molecular Genetics 1h 17m 04s
Module-12(BC-C02-M12): Fundamental of Pharmacogenomics

Fundamental of Pharmacogenomics 1h 02m 46s
Module-13(BC-C02-M13): Pharmacogenomics in clinical practice and Future of Pharmacogenomics

Pharmacogenomics in clinical practice and Future of Pharmacogenomics 1h 01m 47s
Module-14(BC-C02-M14): Fundamental of Precision Medicine, Precision Medicine in

Fundamental of Precision Medicine, Precision Medicine in Clinical Practices and The Future of Precision Medicine 1h 01m 15s
Module-15(BC-C02-M15): Course Final Assessment

Course Final Assesment 0h 10m 00s

Bundle Course (3 in 1) : RT-PCR: Techniques and Applications + Conventional PCR with Gel Electrophoresis Technique+ Pharmacogenomics Screening

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0 Enrolled

15 Lessons

Duration : 15:07:34

20000.00 ৳

Bundle Course (3 in 1) : RT-PCR: Techniques and Applications + Conventional PCR with Gel Electrophoresis Technique+ Pharmacogenomics Screening

Course Description

Elevate your expertise in molecular biology with our comprehensive bundle course, meticulously designed to provide both theoretical knowledge and practical skills. This program encompasses:

Hands-on Training in RT-PCR: Delve into the principles and applications of Reverse Transcription PCR, mastering techniques essential for molecular diagnostics.
Conventional PCR with Gel Electrophoresis: Gain practical experience in DNA amplification and visualization, understanding the nuances of gel electrophoresis for DNA fragment analysis.
RT-PCR based Pharmacogenomics & Precision Medicine: Explore the intersection of genetics and pharmacology, learning how RT-PCR facilitates personalized medicine by tailoring treatments based on individual genetic profiles.

By the end of this course, participants will be equipped to apply advanced molecular techniques in research, clinical diagnostics, and the burgeoning field of personalized medicine.

Course Outcomes

1. Theoretical Knowledge

Comprehend PCR Fundamentals: Understand the principles, mechanisms, and applications of both Conventional PCR and Real-Time PCR (RT-PCR), including their roles in molecular diagnostics.
Differentiate PCR Techniques: Distinguish between Conventional PCR and RT-PCR methodologies, recognizing their respective advantages, limitations, and appropriate use cases.
Understand Gel Electrophoresis: Grasp the principles of agarose gel electrophoresis and its application in analyzing PCR products.
Explore Pharmacogenomics: Gain insights into pharmacogenomics, understanding how genetic variations influence drug responses and the importance of personalized medicine.

2. Practical Skills

Execute PCR Protocols: Perform DNA and RNA extraction, set up PCR reactions, and operate thermal cyclers for both Conventional PCR and RT-PCR.
Conduct Gel Electrophoresis: Prepare and run agarose gels to separate and visualize DNA fragments, interpreting band patterns to assess PCR results.
Design Primers: Design specific primers for target genes, optimizing annealing temperatures and reaction conditions for efficient amplification.
Implement Pharmacogenomic Testing: Apply RT-PCR techniques for pharmacogenomic screening, identifying genetic markers relevant to drug metabolism and response.

3. Analytical Abilities

Interpret Amplification Data: Analyze amplification curves and Ct values in RT-PCR to quantify gene expression levels and detect genetic variations.
Troubleshoot Experiments: Identify and resolve common issues in PCR experiments, such as non-specific amplification, primer-dimer formation, and weak signals.
Ensure Quality Control: Implement quality assurance measures and standard operating procedures to maintain the integrity and reliability of molecular diagnostic tests.

4. Professional Competence

Apply Techniques in Real-World Scenarios: Utilize acquired skills in research, clinical diagnostics, and biotechnology industries, contributing effectively to laboratory operations.
Adhere to Ethical Standards: Demonstrate professional conduct and ethical considerations in molecular biology practices, including data handling and reporting.
Embrace Precision Medicine: Integrate pharmacogenomic data into clinical decision-making, tailoring treatments based on individual genetic profiles to enhance therapeutic efficacy and safety.

Course Faq

What is the structure of the course?

The course is a hybrid program combining both theoretical and practical components. It includes: Online Theory Sessions: Three days of interactive online lectures covering the fundamentals of PCR techniques. Hands-On Laboratory Practicals: Two days of in-person laboratory sessions focusing on DNA/RNA extraction, PCR setup, and gel electrophoresis procedures.

Who is the target audience for this course?

This course is designed for: Undergraduate and postgraduate students in biotechnology, microbiology, or related fields. Laboratory technicians and research assistants seeking to enhance their molecular biology skills. Healthcare professionals interested in molecular diagnostics. Individuals aiming to acquire practical experience in PCR and gel electrophoresis techniques.

Are there any prerequisites for enrollment?

A basic understanding of molecular biology concepts is recommended. However, the course is structured to accommodate beginners, with foundational topics covered in the initial sessions.

What will I learn during the course?

Comprehensive knowledge of Conventional PCR and Real-Time PCR (RT-PCR) methodologies. Hands-on experience in DNA/RNA extraction, PCR setup, and gel electrophoresis. Skills in data interpretation, including amplification curves and Ct values in RT-PCR. Troubleshooting techniques for common PCR-related issues.

Will I receive a certificate upon completion?

Yes, participants who successfully complete the course will receive a certificate accredited by Skillcare.xyz, acknowledging their proficiency in PCR and gel electrophoresis techniques.

What is the duration and schedule of the course?

Total Duration: One week. Real Compressions Fast CPR & Training +14 American Academy of CPR and First Aid +14 Red Cross +14 Schedule: Three days of online theory sessions followed by two days of in-person laboratory practicals. Specific dates and times will be communicated upon enrollment.

Where are the laboratory sessions conducted?

The hands-on laboratory sessions are held at the Skillcare.xyz Training Facility located in Dhaka, Bangladesh.

What is the course fee?

The total fee for the course is ৳6,000, which includes access to all online materials, laboratory sessions, and the certification upon completion.

How can I enroll in the course?

Enrollment can be completed through the Skillcare.xyz website. Visit www.skillcare.xyz and navigate to the course section to register.

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