Bundle Course (2 in 1): RT-PCR: Techniques and Applications + Conventional PCR with Gel Electrophoresis Technique
Bundle Course (2 in 1): RT-PCR: Techniques and Applications + Conventional PCR with Gel Electrophoresis Technique
Course Description
Elevate your molecular biology expertise with our integrated course combining:
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Hands-on Training in RT-PCR: Delve into the principles and applications of Reverse Transcription PCR, mastering techniques essential for molecular diagnostics.
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Conventional PCR with Gel Electrophoresis: Gain practical experience in DNA amplification and visualization, understanding the nuances of gel electrophoresis for DNA fragment analysis.
This comprehensive program is designed for students, researchers, and professionals aiming to acquire practical skills in PCR methodologies and their applications in diagnostics and research.
Course Outcomes
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Understand the principles and workflow of both Conventional PCR and RT-PCR.
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Perform DNA and RNA extraction from biological samples.
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Conduct cDNA synthesis and gene amplification using RT-PCR.
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Set up and run conventional PCR and analyze results through gel electrophoresis.
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Analyze and interpret amplification plots, melt curves, and gel bands.
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Apply molecular techniques in diagnostic and research settings.
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Learn essential troubleshooting and contamination prevention.
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Improve documentation and lab reporting skills.
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Receive a combined certification in both PCR methodologies.
Course Faq
What is RT-PCR?
RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a technique used to detect and quantify RNA by converting it into DNA and amplifying specific genetic targets in real-time.
What will I learn in this training?
You’ll gain hands-on experience in: RNA extraction cDNA synthesis Real-time PCR setup and amplification Data analysis (Ct values, melt curves) Applications in diagnostics, gene expression, and research
Who can attend this course?
Ideal for: Life science students Research scholars Lab professionals Diagnostic and healthcare workers Anyone interested in molecular diagnostics
Do I need prior lab experience?
No. Basic biology or molecular genetics knowledge is helpful, but beginners are welcome. The training is fully guided.
What are the key benefits?
Learn a high-demand lab skill Get hands-on exposure to real-time PCR machines Improve your diagnostic and research capabilities Receive a certificate upon completion
Hardson Smith
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